Quality Assurance Software: Multiplex_QA
The Multiplex_QA system works in conjunction with the NIH-developed BatchExtract system to help users visualize short- and long-term changes in ABI 310 and 3100 electropherogram quality.
BatchExtract converts ABI binary .fsa files into readily usable text data. Multiplex_QA then uses these files to estimate and display quality metrics that capture changes in electropherographic resolution and efficiency. These metrics mostly are based on the behavior of the internal sizing ladder included with every sample. Multiplex_QA can help practitioners define instrument performance over time.
Several different graphical displays enable users to identify unusual events over time scales ranging from a single .fsa file to all .fsa files from a given instrument.
Download Multiplex_QA
Note: These are large files. You may Email us for CD-ROM of these files.
Downloads
- Software Program (~300 kb WinZip file)]
- User's Manual (~7 Mb WinZip files)
- Example Dataset (~30 Mb WinZip files)]
See Also
- Poster presented at NIJ DNA Grantees meeting June 28, 2005 (Adobe PDF); Duewer, D.L. and Butler, J.M. (2006)
- MULTIPLEX_QA: an exploratory quality assessment tool for multiplexed electrophoretic assays [preprint](Adobe PDF).Electrophoresis, in press.
This Software was developed by the NIST Forensics/Human Identity Project Team with funding from the National Institute of Justice through the NIST Office of Law Enforcement Standards.
Disclaimer: These software programs were developed for use in a research environment and are not guaranteed to work on all possible computer configurations.
